Lentivirus相关论文
Retinol dehydrogenase 10 promotes metastasis of glioma cells via the transforming growth factor-β/SM
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The experimental study of genetic engineering human neural stem cells mediated by lentivirus to expr
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目的 研究慢病毒介导生存素基因（Lentivirus-Survivin）对早、晚期反分化人椎间盘髓核细胞的生物学效应。方法应用组织块法分离培养人......
目的观察慢病毒介导的神经元特异性生长抑素(somatostatin，SST)过表达对大鼠海马电点燃癫痫的抑制作用。方法成年Sprague-Dawley (S......
目的 建立稳定抑制β-eatenin基因表达的入神经母细胞瘤BE2C细胞株,为探讨Wnt/-eatenin信号在神经母细胞瘤发生中的作用提供细胞模......
目的探讨慢病毒介导的热休克蛋白70（HSP70）表达对缺血/缺氧神经细胞细胞膜钙离子通道的影响及其机制。方法取对数生长期的嗜铬细胞瘤......
Objective: The aim of the study was to investigate the interference and anti-tumor effects of lentiviral vector of miRNA......
目的 探讨视网膜母细胞瘤结合蛋白2(retinoblastoma binding protein 2,RBP-2)在人脂肪基质细胞(human adipose-derived stromal c......
目的 构建ADAM10基因RNA干扰重组慢病毒,观察其在N型钙粘蛋白底物加工过程中的重要性,为探求该加工途径在维持心肌组织结构形态和......
目的应用慢病毒介导的RNA干扰技术，对该技术能特异性的抑制黑色素瘤细胞转移进行研究。方法以人巢蛋白(Human nestin)的信使RNA编码......
目的 研究靶向增殖诱导配体(a proliferation-inducing ligand,APRIL)基因的短发夹RNA(short hairpin RNA,shRNA)在体内外对胰腺癌......
背景 视网膜新生血管性疾病是一种严重影响视力的眼病.研究表明,cathepsin B参与新生血管的生成,寻找抑制视网膜新生血管形成的药......
目的 观察超级白细胞介素-6重组慢病毒对人正常肝细胞L-02的促增殖作用.方法 利用慢病毒表达质粒及其包装系统构建超级白细胞介素-......
目的 探讨沉默Notch1基因对人神经胶质瘤U251细胞增殖能力的影响.方法 采用Notch1-shRNA慢病毒感染人胶质瘤U251细胞,筛选稳定低表......
目的探讨微小RNA155(microRNA-155,miR-155)在肝细胞癌(hepatocellular carcinoma,HCC)对索拉非尼(sorafenib)抗药中的作用。方法......
目的探讨在不同脑区(齿状回、海马CA1和内嗅皮质)注射慢病毒对大鼠海马齿状回部位神经元的转染效果。方法健康雄性Sprague Dawley......
目的探讨鞘内注射热敏感TRPV3离子通道的小分子干扰RNA(siRNA)慢病毒对骨癌痛大鼠疼痛行为的影响。方法鞘内成功置管雌性Sprague-D......
Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on
In the present study, we constructed a lentivirus, FIV-CMV-GFP-miR-7-3, containing the microRNA-7-3 gene and the green f......
目的 探讨慢病毒介导的agggrecanase-2 shRNA对类风湿关节炎患者软骨细胞aggrecan的影响.方法 术中切取类风湿关节炎患者关节软骨,......
目的观察重组慢病毒Lv-hTERTp-TK与Lv-hTERTp-tumstatin在肝癌HepG2细胞中的靶向表达，探讨二者联合在体内外对HepG2细胞的抑制作用......
目的检测利用慢病毒介导人β-防御素3 （humanβ-defensin 3, hβD-3）基因转染人滑膜间质干细胞（synoviumderived mesenchymal stem ce......
目的探讨水通道蛋白9（AQP9）对人肝癌细胞系HepG2增殖、凋亡和侵袭迁移的影响。方法构建靶向AQP9基因的慢病毒过表达载体,转染HepG2细......
目的 观察慢病毒介导特异性短发夹RNA (short hairpin RNA,shRNA)干扰第10号染色体同源丢失性磷酸酶张力蛋白基因(phosphatase and......
The experimental study of genetic engineering human neural stem cells mediated by lentivirus to expr
Objective:To explore the feasibility to construct genetic engineering human neural stem cells (hNSCs)mediated by lentivi......
Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma
Background In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Inc......
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Retinol dehydrogenase 10 promotes metastasis of glioma cells via the transforming growth factor-β/SM
Background:Glioma is the most common primary malignant tumor in the central nervous system.Because of the resistance of ......
Construction and Characterization of Lentiviral shRNA Expression Vector Targeting Rat CD40 Gene in D
本文通过对荣华二采区10...
Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression
本文通过对荣华二采区10...
目的 探讨脂素(Lipin1)对糖尿病(DM)大鼠周围神经早期病变的影响.方法 应用高糖高脂饮食结合腹腔注射链脲佐菌素构建2型糖尿病(T2D......
目的：构建精氨酸甲基转移酶2( PRMT2)慢病毒表达载体。方法通过PCR扩增PRMT2 cD-NA,将PRMT2 cDNA连接于GV308载体,经测序确认后,将G......
目的构建稳定敲低E6AP表达的胃癌细胞BGC-823,并检测对胃癌细胞增殖和迁移等恶性生物学行为的影响。方法采用Western印迹方法检测E......
Objective: To construct a lentiviral expression vector for RNA interference (RNAi) of human VIM gene; and assess its gen......
Lentivirual vector-mediated doxycycline-inducible iASPP gene targeted RNA interference in hepatocell
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
GENETIC ENGINEERING NEURAL STEM CELL MODIFIED BY LENTIVIRUS FOR REPAIR OF SPINAL CORD INJURY IN RATS
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
目的 构建大鼠β防御素2(rBD2)基因RNAi慢病毒重组载体,转染培养细胞,检测其沉默效应,筛选出最佳的RNAi慢病毒载体.方法 序列软件......
目的 构建针对人胰腺癌细胞株CFPAC1增殖诱导配体(a proliferation-inducing ligand,APRIL)基因的shRNA慢病毒表达载体.方法 应用......
慢病毒载体感染的宿主广,不仅可以高效感染分裂期细胞,而且可以感染非分裂期细胞,可作为肿瘤免疫治疗的工具.与其他逆转录病毒载体......
目的 研究以慢病毒为载体靶向人乳头瘤病毒16型(HPV16)E6基因的短发夹状干扰RNA(shRNA)对宫颈癌Caski细胞侵袭能力的影响.方法 将......
Lentiviral-mediated vascular endothelial growth factor 165 gene transfer into neural stem cells prom
We constructed a lentiviral vector carrying vascular endothelial growth factor 165,which was used to transfect neural st......
Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carot......
Objective: To construct a lentiviral expression vector for RNA interference (RNAi) of human VIM gene; and assess its gen......
Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on
In the present study, we constructed a lentivirus, FIV-CMV-GFP-miR-7-3, containing the microRNA-7-3 gene and the green f......
Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchy
microRNAs (miRNAs) play an important regulatory role in the self-renewal and differentiation of stem cells. In this stud......
目的 探讨重组慢病毒介导的基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)基因同时沉默对喉鳞癌侵袭生长的抑制作用.方法 采......
目的 构建表达大鼠Cdh1基因的重组慢病毒载体.方法 根据大鼠Cdh1基因的核苷酸序列,合成目的基因片段并亚克隆到慢病毒表达载体pGC-......
目的 通过研究串珠素shRNA慢病毒转染对小鼠胚胎成纤维细胞(NIH3T3)增殖能力的影响,探讨其在抑制硬膜外瘢痕形成中的作用和机制.方......
